Project Description: The aim of this research is the development of EIS methods for the non-destructive estimation of fruit quality evolution during its on-plant and post-harvest ripening. The project focuses on three interconnected research areas:
► The development of a handheld impedance analyzer, with a focus on the size and cost reduction and on the enhancement of the portability of the system, to obtain on-field measurements comparable with the commercially available bench-top instruments. ► The development of custom-made, biocompatible and non-destructive contact electrodes using a combination of printing techniques (i.e. spray coating, screen printing, dispense printing, etc…) on flexible and stretchable substrates (PET, Kapton, PDMS, Fabric). ► The analysis of the impedance output, in terms of its general evolution, influence of the frequency range and the fitting on equivalent circuits. Furthermore, the application of machine learning algorithms for the correlation of the fruit quality parameters with the impedance data will be evaluated.Project Description: The project aims at the development of low-cost electrochemical biosensor for the detection of food toxicants (Mycotoxin, Heavy Metals and Antibiotic residuals) from food products. It mainly involves the following activities:
► Development of electrochemical, ion-selective membrane and molecularly imprinted polymer based biosensors. ► Detection of mycotoxin (AFM1 and OTA), heavy metal and antibiotic residuals from different food sources on a real-time basis. ► Low and improved limit of detection, high sensitivity and selectivity due to use of specific antibodies and selective membrane using nanomaterials.Project Description: The aim of this research is interfacing biology and electronics, in order to develop low-cost printed biosensors for fast detection of food-borne hazards. This project focuses on detection and quantification of biogenic amines, as their presence in food constitutes a potential public health concern due to their physiological and toxicological effects.
Biosensors, fabricated with printing techniques (such as screen printing, inkjet printing, dispense printing, 3D printing) on mechanically flexible substrates, functionalized with nanoparticles (such as carbon nanotubes, silver nanoparticles etc.) and immobilized with antibodies for specific detection are promising analytical tools for fast and low-cost analysis. Furthermore, selectivity, reproducibility, mechanical and time stability need to be evaluated to be able to implement biosensors in the food safety analysis and moving toward industrial-scale manufacturing.
Main research areas includes:
► Flexible and printed biosensor ► Immunosensors ► Simultaneous detection of food hazardsProject Description: Currently food waste is a major problem (30% of all food produced is wasted) and 50% of this waste is produced when the food is packed. This waste is mainly due to the oxidation of lipids and proteins and microbial spoilage. During the food spoilage, the food starts releasing gases (NH3, TMA, and DMA). By detecting the presence of these three gases we can detect the spoilage at the initial stage when the food is still consumable. Therefore, if we want to reduce the food waste, we have to increase the shelf-life of food products by reducing the spoilage rate (oxidation and microbial spoilage), thus reducing these two phenomena is mandatory. In order to achieve this:
► We will develop a gas sensor array for NH3, TMA, and DMA detection which will monitor the headspace of the packaging and coat the inner-side of the package with smart materials incorporated with antioxidant and antimicrobial agents. ► Then when the presence of the analyte is detected the sensor will send an electrical signal to the smart materials. ► Under the electrical stimulation induced by the sensor the volatile agents loaded inside will be realized and stops or reduce the spoilage rate of the food. In this way the sensor and the smart materials they work together in a closed system and extend the shelf life of the food product as long as possible.Project Description: The aim of this research project, conducted in collaboration with the researchers of the Center of Materials and Microsystem (CMM) at Fondazione Bruno Kessler (FBK), is to develop a flexible and printed gas, temperature and humidity sensor employing different printing techniques suitable for the deposition on flexible substrates, and to finally build a flexible printed sensors array. Different prototypes of the sensors will be developed and characterized, focusing on metal oxide (MOx)-coated carbon nanotubes (CNTs) as a sensing material. The sensing material is deposited on microheaters developed at FBK in Trento using spray deposition, whereas the CNTs are coated with the MOx using magnetron sputtering. The sensitivity of different materials and preparations will be studied in relation to sensitivity to different gases.
Project Description: This Ph.D. thesis project aims at fabricating carbon nanotube-based field-effect transistor (CNTFET) biosensors on flexible substrates to detect typical additives used in industrial beverages and wine. In particular, CNTFETs will be first designed and fabricated using different deposition techniques and patterning steps. Subsequently, to develop biosensors sensitive to the abovementioned analytes, selective and specific enzymes will be immobilized and used to react with the analytes. Characterization of material morphology and electrical performance will be observed on both CNTFETs and CNTFET-based biosensors. As a final goal of the project, a CNTFET biosensor array will be developed to monitor the different additives at the same time on a real-time basis. This biosensor array will help to detect all these carcinogenic additives with low detection limit, allowing broad-range employment in the field of food quality monitoring in the beverage sectors.
Project Description: This project aims to develop a carbon nanotube-based (CNTs) chemiresistive sensor to monitor the ammonia (NH3) gas produced by the gut microbiota inside the colon bioreactors in in-vitro systems. The human gastrointestinal (GI) gases are closely related to diverse pathological disorders, and therefore can be used as a potential health assessment tool. Additionally, GI gases can be correlated with other important bacteria fermentation byproducts such as Short Chain Fatty Acids (SCFA), which are the main indicators of microbial activity and functionality. The advantage of using CNTs gas sensors instead of bulky and costly analytical techniques is that they are low cost, easy to fabricate, and can work at room temperature in real-time. The aim of the project is divided into the following objectives:
► Development and fabrication of carbon nanotubes based NH3 sensors using different printing techniques
► Development and characterization of carbon nanotubes based NH3 sensors on a simple Simulator of the Human Microbial Ecosystem (SHIME) in collaboration with the Micro4Food lab